Journal: Micromachines

Article Title: Improving Single-Cell Encapsulation Efficiency and Reliability through Neutral Buoyancy of Suspension

doi: 10.3390/mi11010094

Figure Lengend Snippet: THP-1 cells maintained high viability with the presence of OptiPrep™ over 24 h inside the droplets. ( A ) Cell viability at two time points: 12 h and 24 h; * represents p < 0.05; † represents no significant difference; error bars represent the standard deviation. ( B ) Images showing cell viability. Measurement of cell viability was performed with the live/dead assay, live cells were stained with Calcein-AM (green) and dead cells with EtD-III (red); scale bar = 100 μm for images obtained by a 20× objective; scale bar = 10 μm for insets obtained by a 100× objective.

Article Snippet: Then, the viability assay kit for animal live and dead cells (Biotium, Hayward, CA, USA) containing 2 μM Calcein AM and 4 μM EthD-III was mixed with the released cells at room temperature for 20 minutes.

Techniques: Standard Deviation, Live Dead Assay, Staining

Journal: Oncotarget

Article Title: TGF-β–independent CTGF induction regulates cell adhesion mediated drug resistance by increasing collagen I in HCC

doi: 10.18632/oncotarget.15521

Figure Lengend Snippet: A . Collagen 1A1 mRNA expression in control (siCont) and COL1A1 knockdown HCC cell lines (siCOL1A1). Expression of mRNA was evaluated by real-time PCR, and the values were normalized to GAPDH. The values of siCOL1A1 were normalized to siCont. B . After transfection of HCC cell lines with siCont and siCOL1A1, the capacity of spheroid formation was evaluated for 5 days. C, D . After 3 days of spheroid formation, Huh7 spheroids were treated with sorafenib (C) or cisplatin (D) for another 4 days. On the final day of treatment (at 7days from cell seeding), spheroids were stained with ethidium homodimer-1 (EthD-1) to evaluate the extent of cell death. After image acquisition, EthD-1 intensity was measured using the same method. The values were normalized to control (0μM) of each group. Data represent the mean values ± SD from two independent experiments. *p<0.05, **p<0.005. Scale bar = 200μm.

Article Snippet: Dead cell was detected using the cell-impermeant viability indicator ethidium homodimer-1 (EthD-1; Invitrogen, Eugene, OR, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Transfection, Staining